am looking for a postdoctoral fellow to work on the cold inactivation of
enzymes. The research entails: structure determination by X ray crystallography,
molecular biology, biophysics and biochemistry. Work is related to the
quaternary structure of proteins, protein-protein interaction, and the role of
hydrophobic interactions in maintaining the quaternary structure of proteins. It
includes enzyme kinetics, HPLC studies of dissociation of oligomric proteins,
calorimetry and high pressure studies. This project is in collaboration with Dr.
Orna Almog from the Clinical Biochemistry Department here, and Prof. Phillips
from Georgia Univ, USA.
What is it that controls bacterial cell division in
space and in time? This is still an enigmatic matter to biologists. We have a
working hypothesis which we try to advance as a possible mechanism for bacterial
cell cycle. We look for a postdoc to approach this problem by studying model
giant liposomes and black membrane interaction with DnaA protein, the protein
which initiated the bacterial genome replication. Techniques to be used in the
research are: single molecule fluorescence—by fluorescence correlation
spectroscopy with confocal microscopy, FRET, homo-FRET and red edge
fluorescence. A strong physical chemistry background is preferred. Work is to be
conducted under the collaborative supervision of Prof. Y. Fishov from the
Department of life Sciences Prof. Oleg Kriechevsky from the Physics Department
and Prof.A.H. Parola form the Chemistry Department.
Abraham H. Parola,
Professor and Dean of the Faculty of Natural Sciences.
H., Ohana, E., Maymon, E., Cohen-Luria, R., Molcho, J., and Parola, A.H.,
Intrinsic fluorescence polarization of amniotic fluid: II. Toward a noninvasive
method for the determination of fetal lung maturity. Photochemistry and
Photobiology, 2006. 82(6): p. 1591-1594.
A., Gdalevsky, G.Y., Cohen-, R., Fishov, I. and Parola, A.H., Membrane-catalyzed
nucleotide exchange on DnaA - Effect of surface molecular crowding. Journal
of Biological Chemistry, 2006. 281(18): p. 12526-12534.
A., Gdalevsky, G.Y., Cohen-Luria, R., Parola, A.H. and Goldgur, Y.,
Crystallization and preliminary X-ray analysis of the apo form of Escherichia
coli tryptophanase. Acta Crystallographica Section D-Biological
Crystallography, 2004. 60: p. 2073-2075.
J., Avraham, H., Cohen-Luria, R. and Parola, A.H., Intrinsic fluorescence
polarization of amniotic fluid: Evaluation of human fetal lung maturity.
Photochemistry and Photobiology, 2003. 78(2): p. 105-108.
S., Pines, D., Pines, E., Parola, A.H. and Fishov, I., Coexistence of domains
with distinct order and polarity in fluid bacterial membranes. Photochemistry
and Photobiology, 2002. 76(1): p. 1-11.